• Accueil et urgences01 64 34 11 55
  • Référer un Cas

Tétralogie de Fallot

Étude clinique sur 31 cas

Sommaire

Tetralogy of Fallot is a complex congenital heart disease that typically consists of a VSD, override of the interventricular septum by the aortic root, obstruction of the right ventricular outflow tract, and secondary hypertrophy of the right ventricle.1,2

The disease is caused by an anterior deviation and abnormal septation of the conal septum during the embryonic period. 3–5

This combination of conotruncal abnormalities was first described in 1888 by Etienne-Louis Arthur Fallot6 as “la maladie bleue” (“blue baby syndrome”).

Auteurs : 

Valérie Chetboul DVM, PhD
Isabelle Pitsch DVM
Renaud Tissier DVM, PhD
Vassiliki Gouni DVM, PhD
Charlotte Misbach DVM, PhD
Emilie Trehiou-Sechi DVM
Amandine M. Petit DVM
Cécile Damoiseaux DVM
Jean-Louis Pouchelon DVM, PhD
Loïc Desquilbet PhD
Éric Bomassi DVM

From the Unité de Cardiologie d’Alfort (Chetboul, Pitsch, Gouni, Misbach, Trehiou-Sechi, Petit, Damoiseaux, Pouchelon), Unité de Pharmacie-Toxicologie (Tissier), and Department of Biostatistics and Clinical Epidemiology (Desquilbet), Université Paris-Est, École Nationale Vétérinaire d’Alfort, Centre Hospitalier Universitaire Vétérinaire d’Alfort, 94704 Maisons-Alfort cedex, France; the Centre Hospitalier Vétérinaire des Cordeliers, 29 avenue du maréchal Joffre, 77100 Meaux, France (Pitsch, Bomassi); and INSERM U955, Equipe 03, 51 avenue du maréchal de Lattre de Tassigny, 94010 Créteil cedex, France (Chetboul, Tissier, Pouchelon). 

Address correspondence to Dr. Chetboul (valerie.chetboul@vet-alfort.fr).
Centre Hospitalier Vétérinaire des Cordeliers, 29 avenue du Maréchal Joffre, 77100 Meaux.
Cet article a été publié dans : JAVMA (15 Oct. 2016) Vol 249 No. 8

Étude clinique sur 31 cas de Tétralogie de Fallot

Objective 

To characterize the epidemiological, clinical, and echocardiographic features of dogs and cats with tetralogy of Fallot (TOF) and determine their survival times.

Design

Retrospective case series.

Animals

15 dogs and 16 cats with a diagnosis of TOF as determined via echocardiography.

Procedures

Medical records of dogs and cats were reviewed to extract information on signalment, clinical status at the time of TOF diagnosis, echocardiographic findings, and any outcome data.

Results

The most common canine breeds were Terrier types (n = 7). Most animals (28/31 [90%]) had clinical signs of TOF at the time of diagnosis, including cyanosis (16/31 [52%]). Pulmonic stenosis was characterized by a variable systolic Doppler-derived pressure gradient (median [range], 108 mm Hg [26 to 255 mm Hg]). Most ventral septal defects were large, with a median (range) ratio of the diameter of the ventricular septal defect to that of the aorta of 0.60 (0.18 to 1.15). Median age at cardiac-related death was 23.4 months, with no significant difference between dogs and cats.
Median survival time from TOF diagnosis to cardiac-related death was briefer for animals with no or low-grade heart murmur (3.4 months) than for those with higher-grade heart murmur (16.4 months). After adjustment for age and sex, having a lack of or a low- to mild-grade systolic heart murmur was significantly associated with a briefer survival time.

Conclusions and clinical relevance

With a few exceptions, cardiac-related death occurred predominantly in young adult dogs and cats with TOF, and most animals had severe clinical signs at the time of TOF diagnosis. (J Am Vet Med Assoc 2016;249:xxx-xxx)

Abbreviations

CI Confidence interval
ΔP Doppler-derived trans-stenotic pressure gradient
HR Hazard ratio
IQR Interquartile range
LVFWs Left ventricular free wall in systole
PT:Ao Ratio of the diameter of the pulmonary trunk to the diameter of the aorta
RVFWs Right ventricular free wall in systole
VSD Ventricular septal defect
VSD:Ao Ratio of the diameter of the ventricular septal defect to the diameter of the aorta

 

Epidemiological, clinical, and echocardiographic features and survival times of dogs and cats with tetralogy of Fallot: 31 cases (2003–2014)

Tetralogy of Fallot has been extensively studied in humans, in whom it is considered the most common congenital cyanotic heart disease, with an incidence of 3 to 5 cases/10,000 live births, accounting for 5% to 10% of all congenital heart diseases.7–9 Several genetic mutations such as those of the genes ZFPM2 and NKX2.5 have contributed to sporadic cases of TOF in humans.4,10

Similarly, TOF is the most common cyanotic heart disease of dogs and cats, accounting for 0.6% to 6.9% of all congenital heart diseases in these 2 species.11–15 The predisposition of Keeshonds to TOF has a suspected genetic basis,16–18 but to the best of the authors’ knowledge, no specific genetic mutation has been identified in dogs.19 Other than the conotruncal malformations reported for Keeshonds,16–18 TOF has been sporadically reported for other canine breeds.20–24 Only a few cases have been reported for cats.11,25–30 Data regarding a series of dogs and cats with TOF are therefore lacking, and survival times of dogs and cats with TOF remain unknown. The purpose of the study reported here was to characterize the epidemiological, clinical, echocardiographic, and Doppler features of dogs and cats with TOF and determine their clinical outcomes and survival times.

Materials and Methods

Medical records review

Paper and electronic medical records were identified of client-owned dogs and cats that had received a conventional (ie, M-mode and 2-D) and standard Doppler echocardiographic examination resulting in a diagnosis of TOF at the Cardiology Unit of Alfort at the National Veterinary School of Alfort, France, and at the Centre Hospitalier Vétérinaire des Cordeliers in Meaux, France, between April 1, 2003, and June 30, 2014. All animals with TOF confirmed by conventional and Doppler echocardiography were included in the study. Animals with equivocal echocardiographic findings were excluded.

Data were obtained from the medical records regarding patient species, breed, age, sex, and body weight. Clinical status at the time of TOF diagnosis, echocardiographic findings (including measurements and presence and types of concomitant congenital heart disease), and any outcome data included in the records were also extracted.

Standard and Doppler echocardiography

Standard 2-D, M-mode, and Doppler echocardiographic examinations were performed while dogs and cats were awake and standing by trained observers at the Cardiology Unit of Alforta and the Centre Hospitalier Vétérinaire des Cordeliers,b as described elsewhere.31,32 Continuous ECG monitoring was provided throughout the examinations. Characteristics of the 4 components of TOF (VSD, overriding aorta, right ventricular outflow tract obstruction, and secondary right ventricular hypertrophy) were assessed via standard parasternal views (Figure 1). Presence of VSD was assessed on right parasternal long-axis 4- or 5-chamber views and by examination of the interventricular septum on right or left longaxis, short-axis, or oblique views.33–35 Transverse views of the heart base, just proximal to the pulmonic valve within the right ventricular outflow tract, were also examined. Color-flow Doppler mode was used to identify and confirm the presence of VSD.33–36 Both color-flow and continuous-wave Doppler modes were used to assess the shunt direction (left-to-right, right-to-left, or bidirectional), as described elsewhere.33–36 Continuouswave Doppler mode was also used to measure the maximal velocity through the defect.

 

Figure 1—Representative M-mode (A), 2-D (B), and color-flow Doppler (C and D) echocardiographic views of a cat with TOF.
A—The M-mode image reveals severe right ventricular myocardial hypertrophy (double-headed arrow), with a high ratio of right ventricular free wall (RVFW) to left ventricular free wall (LVFW) thickness (1.43).
B—The 2-D left parasternal short-axis view at the level of the aortic valve reveals severe hypoplasia of the pulmonary trunk (arrows), characterized by a PT:Ao of 0.52 without any poststenotic dilatation. Notice also the enlarged right atrial (RA) chamber. This cat was characterized as having a high ΔP value (169 mm Hg) as assessed by continuous wave Doppler mode.
C—A right parasternal 5-chamber Doppler view shows the left-toright component of a bidirectional shunt through a large VSD, located just below the overriding aortic root.
D—Another right parasternal 5-chamber Doppler view shows the right-to-left component of the same bidirectional shunt.
Ao = Aorta. IVS = Interventricular septum. LA = Left atrium. LV = Left ventricle. PT = Pulmonary trunk. RV = Right ventricle.

Maximal size of the defect (ie, VSD diameter) was measured from Doppler color-flow map echocardiograms at the level of the defect through the interventricular septum.33,35 As described elsewhere,35 color-flow Doppler images were analyzed frame by frame to compute the maximum area of the VSD jet signal. The largest color-flow VSD diameter was then measured. Lastly, the transverse aortic diameter was measured along the commissure between the noncoronary and left coronary aortic valve cusps in late diastole by use of a 2-D method, as described elsewhere.31,32 The VSD:Ao was then calculated.

Right parasternal long-axis 5-chamber echocardiographic views were also used to confirm override of the aorta, which was defined as a displacement of the aorta to the right so that it appeared to have a biventricular connection overriding the muscular ventricular septum.2 Right ventricular outflow tract obstruction and obstructive abnormalities of the pulmonic valve and pulmonary trunk (all hereafter referred to as pulmonic stenosis) were diagnosed by means of right and left parasternal short-axis views at the level of the aortic valve. Morphological features of pulmonic stenosis (valvular, subvalvular, and supravalvular obstruction) were assessed from the same views by use of 2-D and color-flow Doppler modes.

Measurements of the diameters of the aorta and pulmonary trunk were obtained by means of a 2-D method and a right parasternal transaortic short-axis view, as described elsewhere.37 Briefly, the end-diastolic diameter of the pulmonary trunk was measured just under the closed pulmonic valve, the aortic diameter was measured on the same view, and the PT:Ao was then calculated. The PT:Ao was considered unremarkable when values in dogs were between 0.80 and 1.15,37 and hypoplasia of the pulmonary trunk was diagnosed when the PT:Ao was < 0.80. A similar cutoff was applied to cats.

Additionally, continuous-wave Doppler mode was used to assess the maximal systolic velocity through the pulmonic stenosis. The modified Bernoulli equation was then applied to calculate the peak ΔP and to grade the severity of pulmonic stenosis. Pulmonic stenosis was considered mild for ΔP values ≤ 50 mm Hg, moderate for ΔP values between 50 and 80 mm Hg, and severe for ΔP values > 80 mm Hg.38

Thicknesses of the RVFWs and LVFWs were measured by means of 2-D-guided M-mode echocardiography on the right parasternal ventricular short-axis view,39 in accordance with the recommendations of the American Society of Echocardiography.40 The ratio of the thickness of the RVFWs to that of the LVFWs was then calculated to assess the severity of right ventricular hypertrophy. Hypertrophy was considered severe when the ratio was ≥ 1.5. Concomitant congenital heart diseases were also recorded.

Follow-up data collection

Follow-up data were collected by a review of the paper and electronic records of only animals with TOF without any other concomitant congenital heart disease. At the time of diagnosis, dogs and cats were classified as clinically or subclinically affected (ie, with or without clinical signs attributed to TOF). Clinical signs for this purpose included exercise intolerance, dyspnea, syncope, and cyanosis. Owners of dogs and cats for which an outcome could not be found in the records at the time the study concluded were contacted by telephone, mail, or email to determine whether their animals were alive (clinically or subclinically affected) or dead (date and cause of death, if known). Dogs and cats for which the outcome could not be obtained at the time the study concluded were considered lost to follow-up and were consequently censored at the time of their last examination on record.

Statistical analysis

Statistical analyses were performed by use of statistical software.c Categorical data are reported as proportions or percentages, and continuous or ordinal data for all variables but survival time are reported as median (range). The Kolmogorov-Smirnov test was used to assess normality of distribution of heart murmur grades and imaging values. Because none of these variables was normally distributed, values were compared between dogs and cats by means of nonparametric Mann-Whitney analysis. Spearman analysis (rs) was used to evaluate correlations between heart murmur grade and ΔP.

Two survival analyses were performed: one for the interval from date of birth to date of cardiac-related death to determine median age at cardiac-related death and the other to assess potential risk factors for cardiacrelated death at the time of diagnosis of TOF. Univariate and multivariate Cox proportional hazard modeling was performed to identify factors associated with survival time. The Kaplan-Meier method was used to estimate median times to cardiac-related death, and these survival times were compared between dogs and cats by use of the log-rank test. Because of the low number of animals, separate multivariate models were developed for each factor hypothesized to be associated with survival time and were systematically adjusted for confounding factors (age and sex). The proportional hazard assumption was checked by including a linear-dependent interaction term, and results (P > 0.13) indicated no deviation from that assumption. Survival times are reported as median (IQR). Values of P < 0.05 were considered significant for all analyses.

Results

Epidemiological features

Fifteen dogs (8 males and 7 females) and 16 cats (11 males and 5 females) with TOF were included in the study, for a total of 31 animals. Twenty-one had received a TOF diagnosis at the Cardiac Unit of Alfort, and 10 had received their diagnosis at the Centre Hospitalier Vétérinaire des Cordeliers.

Dogs were of 9 breeds, with Terrier types predominating (n = 7; 3 Jack Russell Terriers, 2 Maltese Terriers, and 2 Border Terriers), followed by French Bulldog (2), Chihuahua (2), and English Bulldog, Boxer, Cane Corso, and Malamute (1 each).
Median body weight of the dogs was 3 kg (6.6 lb), with a range of 0.5 to 15.2 kg (1.1 to 33.4 lb).

Cats were of 5 breeds, with domestic shorthair predominating (n = 12). Other cat breeds included Persian, Ragdoll, Siamese, and British Blue (1 each).
Median body weight of the cats was 3.2 kg (7.0 lb), with a range of 1.2 to 5.2 kg (2.6 to 11.4 lb).

Overall median (range) age at the time of TOF diagnosis was 7.0 months (1.7 to 54.0 months).
Median (range) age at time of TOF diagnosis for dogs specifically was 3.6 months (1.7 to 49.4 months) and for cats was 9.8 months (2.9 to 54.0 months).

Clinical features

One dog (French Bulldog) and 1 cat (Persian) had concomitant congenital heart disease, consisting for both of an atrial septal defect and subaortic stenosis; values of ΔP were 64 mm Hg and 38 mm Hg, respectively. The remaining 29 (94%) animals had no heart disease other than TOF.

Data obtained from cardiac auscultation at the time of TOF diagnosis were available for 27 (87%) animals (12 dogs and 15 cats). A heart murmur was detected in most (n = 25 [93%]) of these animals. A left systolic basal or midthoracic heart murmur (median grade, 4 [2 to 6]) was detected in 24 (89%) animals, either alone (n = 17 [71%]) or associated with another heart murmur (ie, right systolic midthoracic heart murmur) with a median grade of 4 (2 to 5; n = 7 [29%]), including the dog and cat with concomitant congenital heart disease. One cat had only a systolic right midthoracic heart murmur (grade 5/6).

Most animals (28 [90%]) had clinical signs of TOF at the time of diagnosis (Table 1). The most common clinical signs were exercise intolerance, observed in all clinically affected animals, respiratory signs (eg, dyspnea and panting), and cyanosis.

Treatment status at the time of TOF diagnosis was known for all animals. Twenty one (68%; 11 dogs and 10 cats) were not being treated, and 10 (32%; 4 dogs and 6 cats) were receiving 1 or a combination of the following drugs that had been previously prescribed by referring veterinarians: benazepril or enalapril (4 cats and 1 dog), furosemide (1 dog and 2 cats), atenolol (2 dogs), pimobendan (1 cat), acetylsalicylic acid (1 cat), and spironolactone (1 cat).

Echocardiographic features

The location of the pulmonary stenosis was known for 28 of 31 (90%) animals (15 dogs and 13 cats). Subvalvular stenosis was identified in 9 (32%) of these animals (2 dogs and 7 cats), valvular stenosis was identified in 8 (29%) animals (5 dogs and 3 cats), and supravalvular stenosis was identified in 1 (4%) animal (1 cat). Both subvalvular and valvular stenosis were identified in 4 of 28 (14%) animals (3 dogs and 1 cat), and both valvular and supravalvular stenosis were identified in 1 (4%) animal (1 cat). Combined subvalvular, valvular, and supravalvular stenosis was identified in 5 (18%) animals (all dogs).

A description of the pulmonary trunk at the time of TOF diagnosis was available for 28 of 31 (90%) animals (15 dogs and 13 cats). Hypoplasia of the pulmonary trunk was present in 10 (36%) animals (5 dogs and 5 cats). Values for PT:Ao were available for 13 (42%) animals (5 dogs and 8 cats), with a median (range) value of 0.62 (0.31 to 1.22) for dogs and 0.82 (0.52 to 1.40) for cats. The difference between species was not significant (P = 0.51). Three dogs and 4 cats had a PT:Ao < 0.80 (reference range for dogs, 0.80 to 1.1537; reference range for cats unknown).

Values for ΔP at the time of TOF diagnosis were available for 22 of 31 (71%) animals (12 dogs and 10 cats), with median of 108 mm Hg (range, 26 to 255 mm Hg) for all animals. The median value for dogs (106 mm Hg [range, 39 to 255 mm Hg]) did not differ significantly (P = 0.62) from the median value for cats (108 mm Hg [range, 26 to 169 mm Hg]). Pulmonic stenosis was considered mild for 1 dog and 2 cats, moderate for 4 dogs and 2 cats, and severe for 7 dogs and 6 cats. No significant (P = 0.24) correlation was detected between heart murmur grade and ΔP (rs = 0.29).

Data regarding the ratio of the thickness of the RVFWs to that of the LVFWs at the time of TOF diagnosis were available for 17 of 31 (55%) animals with TOF (9 dogs and 8 cats), with a median (range) value of 1.42 (0.89 to 1.94). No significant (P = 0.67) difference in median ratio values was identified between dogs (1.34 [range, 1.25 to 1.94]) and cats (1.42 [range, 0.89 to 1.88]). All 9 dogs and most (6) cats had a ratio > 1, and 7 animals (4 dogs and 3 cats) had severe right ventricular hypertrophy characterized by a ratio > 1.5 (1.67 [range, 1.56 to 1.94]) and (1.79 [range, 1.51 to 1.88]) for dogs and cats, respectively).

A unique perimembranous and membranous VSD was detected in all animals. Data regarding VSD diameter, assessed by color-flow Doppler mode, were available for 26 (84%) animals (12 dogs and 14 cats), with median value of 6.1 mm (range, 1.7 to 15.0 mm). No significant (P = 0.64) difference in median values was identified between dogs (7.7 mm [range, 3.7 to 11.4 mm]) and cats (5.4 mm [range, 1.7 to 15.0 mm]). The median (range) VSD:Ao calculated for 24 of 31 (77%) animals (10 dogs and 14 cats) was 0.60 (0.18 to 1.15), with no significant (P = 0.64) difference between dogs (0.60 [range, 0.35 to 1.02]) and cats (0.58 [range, 0.18 to 1.15]). Twenty one of 24 (88%) animals (9 dogs and 12 cats) had a VSD:Ao > 0.40, and 17 (71%) animals (7 dogs and 10 cats) had a VSD:Ao ≥ 0.50.

The direction of the shunt, assessed by use of combined color-flow and continuous-wave Doppler modes, was available for 29 of 31 (94%) animals (15 dogs and 14 cats). This direction was bidirectional for 15 (52%) of these animals (7 dogs and 8 cats), left-to-right for 7 (24%) animals (3 dogs and 4 cats), and right-to-left for 7 (24%) animals (5 dogs and 2 cats). No animals with left-to-right shunt were cyanotic. All but 1 animal had clinical signs at the time of diagnosis, including exercise intolerance (2 dogs and 4 cats), dyspnea or panting (1 dog and 2 cats), weight loss (1 dog), and slow growth rate (1 dog and 1 cat).

Cardiac events and survival time

Two animals with TOF (1 French Bulldog and 1 Persian cat) were excluded from subsequent statistical analyses because of other concomitant congenital heart diseases (subaortic stenosis and atrial septal defect for each). The dog was euthanized at 75.4 months of age because of right-sided congestive heart failure. The cat died suddenly at 12 months of age.

Of the remaining 29 animals (14 dogs and 15 cats), 25 received a follow-up examination after TOF diagnosis. Eighteen (72%) of these animals remained untreated, and treatment of the other 7 (28%) animals remained unchanged. Hydroxyurea was occasionally prescribed by the referring veterinarian for 1 dog, and phlebotomy in response to Hct values was performed for another dog.

For all dogs that died during the study period (n = 9), death was related to cardiac events at between 3.5 and 92.9 months of age. Cause or nature of death was spontaneous (associated with worsening of clinical signs such as exercise intolerance, respiratory distress, and inability to remain in the standing position) for 4 dogs, euthanasia for 2 dogs, sudden for 2 dogs, and pulmonary embolism for 1 dog. Death was related to cardiac events at between 6.2 and 83.5 months of age for 10 of the 11 cats that died during the study period. Cause or nature of death was spontaneous (associated with exercise intolerance, tachypnea with marked cyanosis) for 4 cats, euthanasia for 3 cats, and sudden for 3 cats. The remaining cat died at the age of 65 months because of infectious peritonitis.

Kaplan-Meier curves of intervals between birth and cardiac-related death revealed that the median age at death for the 29 animals was 23.4 months, with no significant (P = 0.30) difference between dogs (13.0 months) and cats (23.4 months; Figure 2). Median survival time after TOF diagnosis was 14.5 months (IQR, 4.4 to 29.5 months), with no significant (P = 0.73) difference between dogs (11.1 months) and cats (15.3 months).

Univariate analysis revealed that animals without any heart murmur or with a low- to mild-grade systolic heart murmur survived for a median (IQR) time of 3.4 months (2.9 to 4.4 months) after TOF diagnosis, compared with 16.4 months (10.6 to 29.5 months) for animals with a higher-grade heart murmur (P < 0.01; Figure 3). The crude HR for this comparison was 14.2 (95% CI, 2.6 to 76.7). Similarly, animals with severe right ventricular hypertrophy had a significantly (P = 0.02) briefer survival time after TOF diagnosis than did animals without this disease characteristic (crude HR, 7.3; 95% CI, 1.4 to 36.6). Males (crude HR, 2.6; 95% CI, 0.9 to 7.6; P = 0.08) as well as animals with cyanosis (crude HR, 2.5; 95% CI, 0.9 to 7.2; P = 0.09) had a briefer survival time after TOF diagnosis than did females or animals without cyanosis; however, these differences were not significant. No significant (P > 0.21) association with survival time was identified for species (dog vs cat), syncope, slow growth rate, presence of severe stenosis, VSD:Ao ≥ 0.5, pulmonary hypoplasia, or shunt direction.

After adjustment for age and sex, lack of or lowto mild-grade systolic heart murmur remained significantly (P < 0.01) associated with a briefer survival time (adjusted HR, 13.8; 95% CI, 2.4 to 80.0) after TOF diagnosis, but the association between severe right ventricular hypertrophy and survival time (adjusted HR, 4.4; 95% CI, 0.8 to 23.5) was no longer significant (P = 0.08).

Discussion

Although some reports11,13,15 describe TOF as the first condition associated with cyanosis in dogs and cats with congenital heart disease, few studies have specifically focused on the natural history of TOF in small animals, and most such studies are case reports or case series with only a limited number of cases.20–30 The present study provided original data regarding the comparative epidemiological, clinical, and conventional and Doppler echocardiographic features of 31 dogs and cats with TOF.

Terrier types were the most common canine breeds in the study, accounting for nearly half of the dogs with TOF. A similar breed predisposition for VSD (20/56 [36%]), either alone (7/26 [27%]) or in combination with other congenital heart diseases, was previously reported by our research group.35 These results are in accordance with those of previous studies,12,41,42 suggesting that Terrier-type dogs are predisposed to conotruncal malformations. However, in contrast to other studies,16–18,20 no Keeshonds, Collies, Shetland Sheepdogs, or Beagles were included in the present study, whereas French Bulldog and Chihuahua were the second most commonly affected breeds. Regarding cats, domestic shorthair breeds were overrepresented, as previously reported.42

In the study reported here, most dogs and cats with TOF (29/31 [94%]) had no other congenital heart disease. In humans, a persistent 4th aortic arch is concomitantly diagnosed with TOF and abnormal coronary arteries in 25% and 4% to 5% of cases, respectively.5,43 In the present study, at least 1 systolic heart murmur related to pulmonic stenosis or VSD was detected in most animals with TOF (25/27 [93%]), with a median grade of 4 (6-point scale), regardless of thoracic region where the murmur was most pronounced. Additionally, the majority (90%) of animals had clinical signs (mainly exercise intolerance, dyspnea, and cyanosis) at the time of TOF diagnosis, thus confirming that TOF has early major physiopathologic consequences in both dogs and cats (at a median age of 4 and 10 months, respectively).

As recommended for human patients, 2-D echocardiography combined with M-mode and color-flow Doppler mode was chosen to confirm the presence of TOF. These combined ultrasonographic techniques are considered as the most sensitive method for noninvasive diagnosis of TOF5,44 in humans, allowing identification of the pulmonary stenosis and overriding aorta, determination of the type and size of the VSD, assessment of right ventricular hypertrophy severity and shunt flow direction, and calculation of ΔP values.

As part of the definition of TOF, pulmonic stenosis was diagnosed in all animals included in the present study. For most of them (64%), pulmonic stenosis was characterized by a single obstruction (subvalvular, valvular, or supravalvular), whereas for others (36%) at least 2 obstructive lesions were identified. In humans with TOF, valvular and supravalvular pulmonic stenosis are the most commonly described types of obstructive lesions.5,43 Conversely to the situation in humans, solitary or combined valvular and subvalvular obstructive lesions predominated in the animals of the present study (36 of all 43 [84%] described obstructions), whereas supravalvular obstructions were less common (7/43 [26%]), which is in accordance with other reports.34,45 

The present study also revealed that hypoplasia of the pulmonary trunk, identified in more than a third of all dogs and cats with TOF, was common. Pulmonic stenosis was considered severe for most included animals (59%), whereas the obstruction was considered moderate or mild for only 27% and 14% of animals, respectively. These results explained, at least in part, the high percentage of animals with clinical signs at the time of diagnosis as well as the high prevalence of cyanosis (52%). In addition, the degree of right ventricular hypertrophy was marked for most animals, as reflected by a median value of 1.4 for the ratio of the thickness of the RVFWs to that of the LVFWs. Although right ventricular hypertrophy is part of the definition of TOF, to the best of our knowledge, no published data exist regarding the severity of such myocardial remodeling, compared with that in the left ventricle.

As was identified in other studies,38,46 a large and unique membranous and perimembranous VSD was detected in all animals in the present study. The large size of the defect was confirmed by VSD:Ao values, which involved normalization of the VSD diameter and allowed comparison of VSD severity between animals of different sizes.35 In dogs and cats with a solitary VSD, a good prognosis can be achieved when the VSD diameter is less than half of the aortic root size.47 Similarly, we previously identified a significant difference in VSD:Ao values between clinically and subclinically affected dogs, with a median value of 0.4 for dogs with clinical VSD.35 In the present study, VSD:Ao values were > 0.4 for nearly 90% of all animals, with a median value of 0.6 and with no difference between dogs and cats. The large size of these defects, together with the high prevalence of severe pulmonic stenosis, would explain the high percentage of clinically affected animals at the time of TOF diagnosis.

All possible types of VSD shunt directions associated TOF have been reported for small animals, 38,45,46,48 without any accurate description of their respective prevalences. In the present study,
a VSD was associated with a left-to-right shunt in only 24% of animals, which was consistent with the overall predominance of severe pulmonic stenosis.

Finally, after exclusion of the 2 animals with other concomitant congenital heart diseases, Kaplan-Meier analysis revealed that the survival time of animals with TOF was fairly brief for dogs and cats alike, with all deaths but 1 during the study period related to a cardiac event. The median age at cardiac-related death was 2 years, and only 25% of the animals survived to 7 years of age. Findings therefore suggested a poor long-term prognosis for most dogs and cats with TOF. Interestingly, lack of or low-to-mild-grade heart murmur (vs moderate-tosevere-grade heart murmur) and severe right ventricular hypertrophy were associated with briefer survival times from TOF diagnosis to cardiac-related death, and after adjustment for age and sex, only lack of or low-to-mild-grade systolic heart murmur remained significant. These results are in accordance with clinical reports showing that the intensity and duration of the systolic ejection murmur vary inversely with severity of subvalvular obstruction in human patients with TOF (which is the opposite in patients with isolated pulmonic stenosis).5

This present study had several limitations, mainly related to its retrospective nature. Values for several conventional (eg, VSD diameter or PT:Ao) and Doppler (eg, ΔP) echocardiographic variables were unavailable for some animals. Additionally, Hct and blood oxygen saturation were not recorded in the medical records. Several animals were also lost to follow-up. The low number of animals included in the study resulted in low statistical power, which may have limited the ability to detect significant associations of certain variables with survival time in univariate and multivariate analyses despite high HR values. For example, although severe right ventricular hypertrophy had an HR of 4.4 after adjustment for age and sex, this association did not achieve significance (P = 0.08). The ability to adjust for other potential confounders was prevented owing to the small sample size, and findings should be interpreted with consideration of this limitation. Lastly, only live animals evaluated at 2 referral clinics were included in the study, excluding all animals that may have died before they could be brought to the clinics.

Despite the aforementioned limitations, the present study provided clinical, imaging, and longterm survival data for dogs and cats with TOF, helping to further characterize this complex congenital heart disease and highlighting its severity, which was reflected in the common combination of severe pulmonic stenosis and large VSD. Most affected dogs and cats had major hemodynamic derangements at the time of diagnosis and died of cardiac causes at a young adult age, with a few exceptions. Additional research is therefore required to develop palliative or curative surgical procedures for treatment of dogs and cats with TOF. The potential genetic basis of TOF also needs to be investigated in some specific breeds such as Terrier-type dogs.

Acknowledgments

This study was not supported by any grant or other funding.

Footnotes

  1. Vingmed System 5, Vivid 5, Vivid 7, and Vivid E9, GE Healthcare Inc, Waukesha, Wis.
  2. Megas and My Lab Twice, Esaote Biomedica, Firenze, Italy.
  3. Systat, version 10.0, SPSS Inc, Chicago, Ill.

References

1. Becker AE, Connor M, Anderson RH. Tetralogy of Fallot: a morphometric and geometric study. Am J Cardiol 1975;35:402–412.
2. Bailliard F1, Anderson RH. Tetralogy of Fallot. Orphanet J Rare Dis 2009;4:2.
3. Dupuis C, Kachaner J, Freedom M, et al. Tétralogie de Fallot. In: Cardiologie pediatrique. 2nd ed. Flammarion, Chevilly-Larue, 1991;327–346.
4. Di Felice V, Zummo G. Tetralogy of Fallot as a model to study cardiac progenitor cell migration and differentiation during heart development. Trends Cardiovasc Med 2009;19:130–135.
5. Webb GD, Smallhorn JF, Therrien J, et al. Congenital heart disease: cyanotic heart diseases: tetralogy of Fallot. In: Braunwald’s heart disease. 9th ed. Saunders-Elsevier: Philadelphia, 2012;1411–1467.
6. Fallot ELA. Contribution à l’anatomie pathologique de la maladie bleue (cyanose cardiaque). Marseille Medical 1888;25:77–93.
7. Hoffman JI, Kaplan S. The incidence of congenital heart disease. J Am Coll Cardiol 2002;39:1890–1900.
8. Egbe AC, Mittnacht AJ, Nguyen K, et al. Risk factors for morbidity in infants undergoing tetralogy of Fallot repair. Ann Pediatr Cardiol 2014;7:13–18.
9. Moller JH, Ray C, Anderson C. A 43- to 54- year follow-up of 1,000 patients with congenital heart disease. Am J Cardiol 2013;111:1496–1500.
10. Svensson EC, Tufts RL, Polk CE, et al. Molecular cloning of FOG-2: a modulator of transcription factor GATA-4 in cardiomyocytes. Proc Natl Acad Sci U S A 1999;96:956–961.
11. Liu SK. Pathology of feline heart disease. Vet Clin North Am 1977;7:323–339.
12. Scansen BA, Cober RE, Bonagura JD. Congenital heart disease. In: Bonagura JD, Twedt DC, eds. Kirk’s current veterinary therapy XV. Philadelphia: WB Saunders, 2014;2038–2049.
13. Tidholm A. Retrospective study of congenital heart defects in 151 dogs. J Small Anim Pract 1997;38:94–98.
14. Riesen SC, Kovacevic A, Lombard CW, et al. Prevalence of heart disease in symptomatic cats: an overview from 1998 to 2005. Schweiz Arch Tierheilkd 2007;149:65–71.
15. Oliveira P, Domenech O, Silva J, et al. Retrospective review of congenital heart disease in 976 dogs. J Vet Intern Med 2011;25:477–483.
16. Patterson DF, Pyle RL, Van Mierop L, et al. Hereditary defects of the conotruncal septum in Keeshond dogs: pathologic and genetic studies. Am J Cardiol 1974;34:187–205.
17. Van Mierop LH, Patterson DF, Schnarr WR. Hereditary conotruncal septal defects in Keeshond dogs: embryologic studies. Am J Cardiol 1977;40:936–950.
18. Patterson DF, Pexieder T, Schnarr WR, et al. A single majorgene defect underlying cardiac conotruncal malformations interferes with myocardial growth during embryonic development:
studies in the CTD line of keeshond dogs. Am J Hum Genet 1993;52:388–397.
19. Lee JS, Hyun C. Genetic screening of the canine zinc finger protein multitype 2 (cZFPM2) gene in dogs with tetralogy of Fallot (TOF). J Anim Breed Genet 2009;126:304–310.
20. Ringwald RJ, Bonagura JD. Tetralogy of Fallot in the dog: clinical findings in 13 cases. J Am Anim Hosp Assoc 1988;24:33–43.
21. Orton EC, Mama K, Hellyer P, et al. Open surgical repair of tetralogy of Fallot in dogs. J Am Vet Med Assoc 2001;219:1089–1093.
22. Pelosi A, Eyster GE, Dejardin LM, et al. Use of a jugular vein graft in a modified Blalock-Taussig procedure in dogs. Am J Vet Res 2006;67:174–179.
23. Brockman DJ, Holt DE, Gaynor JW, et al. Long-term palliation of tetralogy of Fallot in dogs by use of modified Blalock-Taussig shunt. J Am Vet Med Assoc 2007;231:721–726.
24. Fukushima R, Yoshiyuki R, Machida N, et al. Extreme tetralogy of Fallot in a dog. J Vet Med Sci 2013;75:1111–1114.
25. Bush M, Pieroni DR, Goodman DG, et al. Tetralogy of Fallot in a cat. J Am Vet Med Assoc 1972;160:1622–1631.
26. Kirby D, Gillick A. Polycythemia and tetralogy of Fallot in a cat. Can Vet J 1974;15:114–119.
27. Eyster GE, Weber W, McQuillan W. Tetralogy of Fallot in a cat. J Am Vet Med Assoc 1977;171:280–282.
28. Miller CW, Holmberg DL, Bowen V, et al. Microsurgical management of tetralogy of Fallot in a cat. J Am Vet Med Assoc 1985;186:708–709.
29. Fossum TW, Miller MW, Rogers KS, et al. Chylothorax associated with right-sided heart failure in five cats. J Am Vet Med Assoc 1994;204:84–89.
30. Fruganti A, Cerquetella M, Beribè F, et al. Clinic and ultrasonographic findings in a cat with Tetralogy of Fallot. Vet Res Commun 2004;28 Suppl 1:343–346.
31. Chetboul V, Concordet D, Pouchelon JL, et al. Effects of inter- and intra- observer variability on echocardiographic measurements in cats. J Vet Med A Physiol Pathol Clin Med 2003;50:326–331.
32. Chetboul V, Athanassiadis N, Concordet D, et al. Observer dependent variability of quantitative clinical endpoints: the example of canine echocardiography. J Vet Pharmacol Ther 2004;27:49–56.
33. Hornberger LK, Sahn JS, Krabill KA, et al. Elucidation of the natural history of ventricular septal defects by serial Doppler color flow mapping studies. J Am Coll Cardiol 1989;13:1111–1118.
34. Boon JA. Congenital shunts and AV valve dysplasia. In: Boon JA, ed. Manual of veterinary echocardiography. 2nd ed. Baltimore: Wiley-Blackwell, 2011;437–475.
35. Bomassi E, Misbach C, Tissier R, et al. Signalment, clinical features, echocardiographic findings, and outcome of dogs and cats with ventricular septal defects: 109 cases (1992–2013). J Am Vet Med Assoc 2015;247:166–175.
36. Otto CM. Echocardiographic evaluation of the adult with unoperated congenital heart disease. In: The practice of clinical echocardiography. 2nd ed. Philadelphia: WB Saunders Co, 2002;884–887.
37. Serres F, Chetboul V, Gouni V et al. Diagnostic value of echo-Doppler and tissue Doppler imaging in dogs with pulmonary arterial hypertension. J Vet Intern Med 2007;21:1280–1289.
38. Boon JA. Stenotic lesions. In: Boon JA, ed. Manual of veterinary echocardiography. 2nd ed. Baltimore: Wiley-Blackwell, 2011;477–525.
39. Thomas WP, Gaber CE, Jacobs GJ, et al. Recommendations for standards in transthoracic two-dimensional echocardiography in the dog and cat. Echocardiography Committee of the Specialty of Cardiology, American College of Veterinary Internal Medicine. J Vet Intern Med 1993;7: 247–252.
40. Sahn DJ, DeMaria A, Kisslo J, et al. Recommendations regarding quantitation in M-mode echocardiography: results of a survey of echocardiographic measurements. Circulation 1978;58:1072–1083.
41. Matic SE. Congenital heart disease in the dog. J Small Anim Pract 1988;29:743–759.
42. Hunt GB, Church DB, Malik R, et al. A retrospective analysis of congenital cardiac abnormalities (1977–1989). Aust Vet Pract 1990;20:70–75.
43. Redington A. Tetralogy of Fallot and pulmonary atresia with ventricular septal defect. In: Moller JH, Hoffman JIE, eds. Pediatric cardiovascular medicine. 2nd ed. Oxford, England: Wiley-Blackwell, 2012;590–605.
44. Otto CM, Schwaegler RG, Freeman RV. Complex congenital heart disease: tetralogy of Fallot. In: Echocardiography review guide: companion to the textbook of clinical echocardiography. 2nd ed. Philadelphia: Saunders-Elsevier, 2011;381.
45. Strickland KN. Congenital heart disease: tetralogy of Fallot. In: Tilley LP, Smith FWK, Oyama MA, et al, eds. Manual of canine and feline cardiology. 4th ed. St Louis: Saunders- Elsevier, 2008;235–236.
46. Ware WA. Congenital cardiovascular diseases: tetralogy of Fallot. In: Cardiovascular disease in small animal medicine. London: Manson Publishing, 2007;247–249.
47. Gompf RE, Bonagura JD. Ventricular septal defect. In: Bonagura JD, Twedt DC, eds. Kirk’s current veterinary therapy XV. Philadelphia: WB Saunders, 2014;2296–2305.
48. Bonagura JD, Lehmkuhl LB. Congenital heart disease: tetralogy of Fallot. In: Fox PR, Sisson D, Moïse NS, eds. Textbook of canine and feline cardiology: principles and clinical practice. 2nd ed. Philadelphia: WB Saunders, 1999;515–518.

Retour

Informations pratiques

CHV des Cordeliers

Retour
  • Lun
    8h - 12h 13h30 - 19h
  • Mar
    8h - 12h 13h30 - 19h
  • Merc
    8h - 12h 13h30 - 19h
  • Jeu
    8h - 12h 13h30 - 19h
  • Vend
    8h - 12h 13h30 - 19h
  • Sam
    8h - 12h 13h30 - 17h30
  • Dim
    Urgences 24h/7j
Retour

Adresse de la clinique

29 - 35 Av. du Maréchal Joffre 77100 MEAUX
retrouvez-nous sur Google Maps